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NG Microsystems Seminar: Shubhra Gangopadhyay, NSF Shubhra Gangopadhyay Super-resolution Imaging of Nanostructures on Plasmonic Chip Advanced super-resolution (SR) techniques rely on expensive, sophisticated, and demanding approaches, such as confocal laser scanning microscopy (CLSM), Airyscanning and ground state depletion (GSD) microscopy. On the other hand, the grating-based surface plasmon resonance (SPR) method can overcome the limit of optical resolution through enhancing and propagating electromagnetic field. In this work, silver nanoparticles (NPs) were imaged utilizing inexpensive silver plasmonic grating platform, fabricated by nanoimprint lithography, with different SR approaches including 3D GSD, Airyscanning and blinking localization microscopy with an epi-fluorescence microscope. In addition, the enhanced fluorescence signal from dye molecules provided a unique ability to observe single-molecule (SM) blinking from 10 nM to 1 fM and lower dye concentrations, as well as, to study the localized effects such as temperature fluctuations, nanoparticle mobility, chemical reactions of nanoenergetics on chip, and bio self-assembly in nanoscal. Biography This Event is For: Graduate • Undergraduate • Faculty • Post-Docs • Alumni |
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